Day 1 :
- Agriculture Engineering | Agriculture & Food Security| Plant Science | Agricultural Production Systems & Agribusiness | Bioresources | Agricultural Biotechnology | Agriculture and Environment | Agroforestry & Landscaping | Livestock Farming & Genetic Engineering
University of Puerto Rico-Mayagüez, USA.
Time : 10:00-10:40
Diego M Viteri is an Assistant Professor in the Department of Agro-Environmental Sciences at University of Puerto Rico. He has more than five years of experience in genetics and breeding for resistance to biotic and abiotic stresses in common bean. Also, he has experience in the development of screening methods to identify crop genotypes with resistance or tolerance to diseases in the greenhouse. He is the author/co-author of more than 20 publications in North American and European journals.
Ashy stem blight (ASB) caused by Macrophomina phaseolina (Tassi) Goidanich (Mp) is an important disease in legumes in the tropics. Resistance has been identified within Cajanus, Phaseolus and Vigna species. Our objectives were to: Evaluate the response of the legumes germplasm conserved at University of Puerto Rico and identify resistant genotypes. 20 common beans were planted in a greenhouse at Isabela; 3 cowpeas, 4 common beans and 42 pigeon peas were planted at Lajas in 2016-2017. PRI16 Mp isolate was inoculated 1 or 3 times at the fourth-internode and lateral branches by the cut-stem method. Evaluation of ASB severity was conducted at 15 and 42 days in Lajas and Isabela, respectively. BAT 477, Othello and Verano common beans were susceptible (mean scores >7) in both locations. I-58-2, ICP 86012, and ICP 98030 pigeon peas were susceptible in Lajas. Partial resistance (scores 4-6) was noted in pigeon pea genotypes: Cortada, Guerrero, ICP 6899 and Pinto Berrocales and Gorda cowpea in Lajas. Similarly, A 195 and Badillo common beans were intermediate in Isabela while PC 50 had partial resistance in both locations. I-8-3-4 and ICP 6915 pigeon peas; PI 339623 (Tanzania) and PI 293570 (Speckled Purplehull) cowpeas were resistant to ASB in Lajas (scores£3). Thus, the cut-stem method was a reliable technique to select genotypes with ASB resistance and should be used in combination with field evaluations. Furthermore, multiple inoculations with the same or different Mp isolates are recommended to identify genotypes with specific and broad-spectrum resistance.
Curtin University, WA.
Title: Economically Viable Aquaponics: Mapping the Gap between Promising Potential and Current Uncertainties
Time : 11:00-11:30
Asael Greenfeld has an MSc in dryland Ecology from BGU Israel and Enviromental Education is Israel and Australia. He is pursuing PhD course in Environmental Economics at Curtin University WA, in conjunction with the Tel-Hai academic college in Israel.
Aquaponics or integrated farming of fish and plants, has potential for sustainably producing high quality food, but has not become a commercial success in most places. In recent years, aquaponics has been receiving growing attention from the scientific community and the current literature covers many aspects of aquaponics production. We reviewed the current literature and classified the specific areas covered by each paper and its contribution to cost reduction or benefit enhancement. Regardless of contradicting views of current profitability, there seems to be a consensus that: Bigger systems are economically superior to smaller ones; profitability is sensitive to retail prices; perhaps most importantly, most authors agree that commercial aquaponics can become more profitable by improving business plans. We identified three under-studied aspects that could each be a game changer for commercial aquaponics: Grower considerations such as financial planning and risk management that may affect potential growers’ initial engagement in aquaponics; Consumer perception of aquaponics products including willingness to pay more for the added value; The economic value of environmental benefits of aquaponic systems and ways to internalise them. We present some results from our study on growers’ and consumers’ perception of aquaponics that highlight the importance of case specific business planning and market research. Further study of each of these three aspects will support ongoing attempts to establish large-scale aquaponics as an economically sustainable practice.
Griffith University, Australia.
Title: Characterisation of R-QTL for Ascochyta lentis resistance in lentil and identification of candidate defence-transcripts
Time : 11:30-12:00
Mahsa is a doctoral student at Griffith University. Her PhD project is on “Identification of genes and molecular defence mechanisms of lentil (Lens culinaris) to Aschochyta lentis a necrotrophic fungus” in Professor Rebecca Ford’s research group. She has been doing various genomics, molecular and plant pathology related studies for the last 10 years. She has lots of experiences on DNA and RNA extraction, PCR and RT-qPCR, gel electrophoresis both on agarose and polyacrylamide gels, RNA and DNA sequencing, Molecular markers, planting and subculturing, disease severity scoring and running large experiments and bioassays. She is currently tutoring in Biological Systems labs at Griffith University along with running the very last PhD related experiments.
Lentil (Lens culinaris) is a valuable staple food legume with an annual global production of ~5MT which is ubiquitously impacted by the disease ascochyta blight, caused by the necrotrophic fungal pathogen, Ascochyta lentis. The most effective and environmentally friendly method to control AB is use of resistant genotypes together with scheduled chemical applications. In order to breed sustainable resistance, knowledge on the key components underpinning the resistance trait is required. Accordingly, a large number of A. lentis defence-related transcripts were identified by RNA-sequencing in the highly resistant genotype ILL7537, which is routinely used in the Australian resistance breeding program . Subsequently, genome-by-sequencing (GBS) method was used to identify SNP markers and produce a comprehensive linkage map comprising of 1161 markers across 120 F5 RIL (ILL7537 x ILL6002 cross). Disease resistance in the RIL population was assessed using four quantitative traits and three major quantitative trait loci governing the ILL7537 resistance were identified (R-QTL). Co-location of differentially expressed defence-related transcripts with SNPs within the identified R-QTL regions revealed a set of putative candidate resistance genes and associated transcription factors, for further investigation as the key drivers of the resistance trait.
University of the Punjab, Pakistan
Time : 12:00-12:30
Zaheer Ahmad Nazar is a PhD Scholar at University of the Punjab, Lahore, Pakistan. He is working on PhD research topic- “Genetic diversity studies for whip smut tolerance in sugarcane germplasm”. He has three research publications.
Determining yield potential of sugarcane lines in terms of their cane weight, height, girth and sugar recovery is essential for their selection in future breeding programs. The performance of 103 promising sugarcane lines/varieties was checked over two successive cropping years under high inoculum conditions for whip smut. During first year of cultivation, 84 sugarcane lines were found highly resistant with only five being susceptible to whip smut. However, 80 sugarcane lines were found highly resistant to whip smut in the ratoon crop. Principal component biplot analysis revealed over all best performance of S.2008-FSD-17, S.2003-US-127, S.2008-US-704 and S.2008-FSD-19 against all the yield traits during both years of plantation while S.2003-US-618 and SPSG-27 showed least performance. Maximum sugar recovery (13%) was recorded in S.2003-US-127, cane weight in S.2011-SL-209 (16.65%), cane height in S. 2008-AUS-190 (3.5 m) and cane girth in SPSG-24 (3.6 cm) while S.2003-US-618 was found highly affected by the whip smut with 74% disease incidence rate among all the studies sugarcane lines/varieties. Further statistical analysis revealed higher estimations of broad sense heritability and genetic advance for the yield traits i.e., cane weight and sugar recovery, which indicated rich potential of using these traits for the development of hybrid and synthetic varieties. These traits were also found more genetically controlled with little to moderate environmental impacts. Moreover, the LSD based ranking of all the sugarcane lines/varieties for their performance against yield traits and smut resistance may help cane breeders in revealing true potential of these sugarcane lines.
Institute of Cotton Research of Chinese Academy of Agricultural Sciences, China.
Title: Genome-wide association mapping for malondialdehyde and proline contents in semi-wild accessions of Gossypium hirsutum races under drought stress
Time : 12:30-13:00
Kiflom Weldu Okubazghi has completed his MSc in Crop Genetics and Breeding from Graduate School of Chinese Academy of Agricultural Sciences. Currently, he is a PhD Scholar at Graduate School of Chinese Academy of Agricultural Sciences. He is also a Lecturer at Hamelmalo Agricultural College, Keren, Eritrea. He has published a paper in a reputed journal named Journal of Integrative Agriculture and his other papers are ready for submission to other reputed journals.
Gossypium hirsutum is the most important source of fiber for textile industry worldwide. To meet the demand of the world’s growing population and rising income, cotton production must be improved. However, crop production, in general, is under threat due to human-mediated climate change, which has left farmers and crop breeders with almost no choice but to contend with frequent and long-term droughts, among other abiotic stress. Semi-wild accessions of Gossypium hirsutum races are repositories of desirable alleles for several economically important traits, such as tolerance to drought stress. Thus, uncovering the molecular basis of drought related traits in the accessions may play a key role in developing new drought tolerant cotton varieties. In this study, to detect single nucleotide polymorphisms (SNPs) significantly associated with drought tolerance related physiological responses, genome-wide association study (GWAS) was conducted in 189 semi-wild accessions of Gossypium hirsutum races using 80K Illumina Infinium SNP array. We performed GWAS using mixed linear model implemented in TASSEL 5 software and detected 1 and 2 SNPs significantly associated with malondialdehyde and proline, respectively on 2 of the 26 cotton chromosomes, explaining 9.80-11.61% of the total phenotypic variation. In addition, we identified 7 and 59 candidate genes related to malondialdehyde and proline, respectively. The identified SNPs and genes not only further advance our understanding of the genetic architecture of drought tolerance but also could be utilized in future drought tolerance improvement programs though marker assisted selection in cotton.
Department of Biotechnology, India.
Title: In silico approaches for structure and function analysis of calcium-dependent protein kinases (CDPKs) in potato (Solanum tuberosum L.)
Time : 13:50-14:20
Gurpreet Kaur is a Research Scholar at Thapar Institute of Engineering and Technology and working in plant molecular biology.
Various stimuli are known to alter the cytosolic concentration of Ca2+, which in turn trigger the physiological responses in plant and animal cells. Such Ca2+-mediated signals are decoded in the form of spatio-temporal expression of different calcium-binding proteins. Calcium-dependent protein kinases (CDPKs) are regarded as one of the largest and most differentiated groups of calcium sensors. The regulatory domains of these proteins bind calcium ions. Activities of the CDPKs are regulated by specific phospholipids and autophosphorylation. CDPK-mediated phosphorylation reactions are known to influence various metabolic activities such as carbon and nitrogen metabolism, phospholipid synthesis, defense responses, ion and water transport, organization of cytoskeletons along with transcription, hormonal responses and intra-cellular signaling. Potato (Solanum tuberosum L.), a member of the Solanaceae family, is a nutritious and non-grain starchy food crop. It is a C3 plant and grows well in short-day and cool night temperature. Multiple allelism is a common feature in the tetraploid potato cultivars. The tubers are the sink organs for storage of starch, proteins and other biomolecules. Tuberization is a complex process that involves number of signals, genes, extrinsic and intrinsic factors and developmentally-regulated metabolic processes. Protein phosphorylation is known to play crucial roles at various stages of tuber development. Several studies revealed the presence of different forms of CDPKs showing varying expression patterns and functional specializations. For example, StCDPK1 is transiently induced upon tuberization in swelling stolons. Likewise, StCDPK3 is involved in tuberization. StCDPK2 is expressed only in leaves; whereas StCDPK4 and StCDPK5 are involved in immune responses. The CDPKs act as mediators of responses to diverse endogenous and environmental cues. The biological function of the individual CDPKs still remain elusive. In this study in silico approaches were adopted for predicting various structural and functional attributes of various potato CDPKs which were hitherto unknown.
Assam Agricultural University, India.
Title: Optimization of surface sterilization protocol and induction of axillary shoots regeneration in Datura metel L.
Time : 14:20-14:50
Rashmi Rekha Boruah has completed her PhD in Department of Agricultural Biotechnology at Assam Agricultural University, Jorhat, Assam, India in 2017. During her PhD, she was accepted as a Visiting Scientist at CSIRO, Canberra, where she conducted research on transgenic chickpea for insect resistance from 2014-2016. She returned to India and conducted research on endophytes with potential acaricidal properties from Datura and Argemone in the DBT-AAU Centre, Assam Agricultural University. She has also published papers in the Indian Journal of Genetics and Plant Breeding for her research on transgenic chickpeas resistant to the pod borer, Helicoverpa armigera.
Micropropagation is an efficient tool for mass propagation of true to type plants. The medicinal values of field grown plants are often affected by biotic and abiotic stresses unlike in vitro regenerated plants. It is important to optimize the conditions for in vitro regeneration in order to avoid microbial contamination and promote the availability of micro propagated plantlets. The objective of this work was to establish an optimized protocol for micropropagation of Datura metel L. Shoot tips from field grown plants were used as explants. Explants were surface sterilized using 1% sodium hypochlorite, 0.1% fungicide (Carbendazim) and 70% ethanol for different exposure timings (1 to 15 minutes). With an increase in exposure time of sodium hypochlorite, contamination of explants declined but blackening of tissues was observed. Among the tested sterilization steps, treatment of explants with 70% ethanol for 1 minute, 0.1% fungicide for 3 minutes and 1% sodium hypochlorite for 10 minutes showed the best result with minimum tissue death. Shoot proliferation was investigated on Murashige and Skoog (MS) basal medium supplemented with different concentrations of 6-Benzylaminopurine (BAP) either alone or in combination with kinetin. Highest multiplication rate was observed on MS medium supplemented with BAP (0.5 mg L-1) and kinetin (0.5 mg L-1).
Institute of Agricultural Information, China.
Title: Modeling of Biomass-Based Leaf Morphological Parameters on Main Stem for Rapeseed (Brassica napus L.)
Time : 14:50-15:20
Wenyu Zhang has completed his PhD from Nanjing Agricultural University. He has published more than 26 papers in journals and has been serving as a Member of computer application in agriculture chapter of Chinese Association of Agricultural Science Societies and The Youth Committee of Jiangsu Society of Systems Engineering.
To quantify the relationships between main stem leaf morphological parameters for rapeseed and the corresponding leaf biomass, field experiments on varieties, transplanting densities and fertilizer were conducted in 2011 to 2012 and 2012 to 2013 seasons. The biomass-based leaf morphological parameter models for rapeseed on main stem were constructed through observing leaf length, maximum leaf width and leaf petiole length under various treatments and analyzing the relationships between leaf morphological parameters and the corresponding leaf biomass. Because both of the leaf length and leaf width were positively proportional to the square root of the corresponding leaf biomass, meanwhile the leaf petiole length was positively proportional to the corresponding leaf length, thus, the changes in the leaf length, the maximum leaf width and the leaf petiole length with the square root of the corresponding leaf biomass for different treatments could be described with linear function. The biomass-based leaf morphological parameter models were validated using independent experiment data and the results showed that the model revealed satisfactory predictions of leaf length, leaf width and leaf petiole length, except the simulation for leaf petiole length of Ningyou 16. The research provides a mechanistic method for linking the rapeseed growth model with the morphological model using organ biomass and lays a foundation for the establishment of functional-structural plant models of rapeseed.
Assessment Institute for Agricultural Technology (AIAT), Indonesia.
Title: The combination of land resource evaluation approach and gis application to determine prime commodities for agricultural land use planning at developed area (a case study of central kalimantan province, indonesia).
Time : 15:20-15:50
Andy Bhermana has completed his PhD from Gadjah Mada University, Indonesia. He is a Researcher of AIAT of Central Kalimantan, Indonesia. He has published more than 20 papers in reputed journals both international and local journals.
Land use planning based on land resource evaluation and spatial orientation of planning as part of GIS may ensures appropriate land allocation in order to achieve sustainable agriculture. The combination of some land resource evaluation can provide final results on rational land allocation and land utilization based on land capability and suitability. Land capability evaluation (LCE) and land suitability evaluation (LSE) approach rooted from USDA and FAO was used in this study to allocated arable land as agricultural developed areas and determine prime commodities spatially. The principle of limiting condition was used for LCE to define land class and determine land availability for agriculture. While, for LSE, procedure of matching between land quality/characteristics and crop requirement will be scored according to limitation method and class criteria of land suitability in order to define the most suitable crop considered as prime commodity. The objective of this study was to determine prime commodities at developed areas on the basis of information of land resources at regional scale of 250,000. With GIS application, the result of evaluation were then integrated into spatial information as basic consideration for agricultural land use planning and further spatial analysis for agricultural land allocation as well. This study was conducted in Central Kalimantan province with total areas of 15,451,287 hectares in order to support policy of Indonesian Government for agricultural development especially for spatial agricultural land use planning. The results of this studies showed that based on LCE approach, almost 61.94% of total areas (9,571,231 hectares) is arable land indicating that lands geographicaly have an opportunity to be developed. While based on LSE approach and GIS application, there are three prime commodities including their geographical distribution pattern that are suitable to be developed in Central Kalimantan involving wetland rubber (7,355,390 hectares), rice (2,141,539 hectares) and oil palm (1,722,806 hectares). Appropriate programs in the scope of land, using planning can then be formulated to support agricultural development at certain areas.
Indonesian Agency for Agricultural Research and Development, Indonesia
Title: Analysis performance of the warehouse receipt system (WRS) to support agricultural marketing in Indonesia
Time : 16:10-16:40
Endro Gunawan has expertise in agricultural economic and public policy. He is evaluating the model of agricultural bio industry where the farming system has zero waste and environmental friendly. He is pursuing his PhD at Asian Institute of Technology (AIT) Thailand major on agribusiness management. He conducts research on agricultural supply chain and the assessment of warehouse receipt system for agricultural commodities in Indonesia.
The common characteristics of agricultural commodities are perishability, seasonality and variability. Agricultural commodity prices fluctuate where during harvest time the price is low. To get a high selling price, farmers can make a "selling delay" by deposit the commodity in the warehouse and re-selling at high prices. By deposit, the goods in the warehouse farmers get a warehouse receipt that can be used as collateral to obtain financing to meet the daily needs as well as for the needs of venture capital. Warehouse Receipt System (WRS) have been implemented in Indonesia in 2008 based on Law No. 9 of 2006 on Warehouse Receipt System. There are ten commodities that can be stored in the WRS warehouse: paddy rice, rice, corn, coffee, cocoa, pepper, seaweed, rattan, rubber, and salt. The implementation of WRS is slow and not widely accepted. The objective of this research are : 1) to analysis the concept of Warehouse Receipt System in Indonesia, and 2) to assess the performance of WRS to support marketing system of corn commodities. The research was conducted in 2015 in Tasikmalaya District, West Java Province. Primary data were collected through direct interviews using structured questionnaires to farmers, trader, WRS Managers and Bank. The data analysed by quantitatively and qualitatively analysis. Data analysis results are presented in the form of analytical tables which then discussed descriptively, comparing the concept and implementation of WRS. The results of the study obtained information that the WRS implemented since 2008 implementation is slow. The benefit is mostly for trader and the procedures is complicated. There are any supporting institution in the WRS such as warehouse managers, assessment agencies (LPK) and financial institutions. The performance of WRS in Tasikmalaya District, West Java Province in 2015 has been utilized by 15 users with the number of corn successfully stored in WRS Jamanis warehouse reached 386.52 tons or equivalent of corn value of Rp 1, 623 billion. The number of WRS proposed as credit collateral amounts to 12 WRS with a total realization of Rp 900 million. The cost component charged from WRS users consist of: the cost for quality test Rp 280.000 per one warehouse receipt, transportation cost Rp. 10-20/kg, drying cost Rp. 200/kg and insurance costs Rp. 0.24 per item value. With the Warehouse Receipt System farmer can expand their marketing channel.