Mohammed Nuruzzaman
National Institute of Agro-biological Sciences, Japan
Title: Rice AP2/EREBP transcription factor family members are differentially expressed in response to six different viruses
Biography
Biography: Mohammed Nuruzzaman
Abstract
Expression profiles of the 147 AP2/EREBP gene family members were studied in rice infected with Rice grassy stunt virus
(RGSV), Rice transitory yellowing virus (RTYV), Rice gall dwarf virus (RGDV), Rice black-streaked dwarf virus (RBSDV),
Rice ragged stunt virus (RRSV), and Rice dwarf virus (RDV). Microarray analysis showed that 80% of the OsAP2/EREBP genes
were differentially regulated during virus infection compared with the control. Rice infected with either RGSV or RBSDV
showed the highest number of upregulated OsAP2/EREBP genes, while RTYV infected plants had the lowest. These results
correlate with the severity of the syndromes induced by the different viruses. Most (60%) of the genes in the RAV subfamily and
B5 subgroup were more highly expressed during RDV-O and RDV-S virus infections, while most (53%) of the genes in the A3
subgroup were down-regulated during RGSV infection, suggesting roles in response to virus infection. The number of genes
activated during RDV infections was greatest during infection with the S-strain, followed by the D84-strain and the O-strain,
with three OsAP2/EREBP genes up-regulated during infection by all three strains. A common set of 12 genes showed higher
expression during infection by at least three viruses, of which, Os05g47650 showed up-regulation during infection with five
of the six viruses. Putative cis-elements related to strongly up-regulated genes included, which may assist in assigning these
important genes to their functional pathways. We also found several duplicate genes that are classified as neo-functional and
sub-functional according to their expression patterns among six virus infections.