Rashmi Rekha Boruah
Assam Agricultural University, India.
Title: Optimization of surface sterilization protocol and induction of axillary shoots regeneration in Datura metel L.
Biography
Biography: Rashmi Rekha Boruah
Abstract
Micropropagation is an efficient tool for mass propagation of true to type plants. The medicinal values of field grown plants are often affected by biotic and abiotic stresses unlike in vitro regenerated plants. It is important to optimize the conditions for in vitro regeneration in order to avoid microbial contamination and promote the availability of micro propagated plantlets. The objective of this work was to establish an optimized protocol for micropropagation of Datura metel L. Shoot tips from field grown plants were used as explants. Explants were surface sterilized using 1% sodium hypochlorite, 0.1% fungicide (Carbendazim) and 70% ethanol for different exposure timings (1 to 15 minutes). With an increase in exposure time of sodium hypochlorite, contamination of explants declined but blackening of tissues was observed. Among the tested sterilization steps, treatment of explants with 70% ethanol for 1 minute, 0.1% fungicide for 3 minutes and 1% sodium hypochlorite for 10 minutes showed the best result with minimum tissue death. Shoot proliferation was investigated on Murashige and Skoog (MS) basal medium supplemented with different concentrations of 6-Benzylaminopurine (BAP) either alone or in combination with kinetin. Highest multiplication rate was observed on MS medium supplemented with BAP (0.5 mg L-1) and kinetin (0.5 mg L-1).